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| Albuquerque,P.P.F.; Cezar,R.D.S.; Pinheiro Junior,J.W.; Grazielle Nascimento,G.; Santos,A.S.; Mota,R.A.. |
| ABSTRACT Paratuberculosis is a chronic and incurable disease that affects ruminants and other domestic animals. It is caused by Mycobacterium avium subsp. paratuberculosis (MAP) that may also be involved in some human diseases such as Crohn's disease, type 1 diabetes, sarcoidosis, multiple sclerosis, and Hashimoto's thyroiditis. The objective of this study was to investigate the occurrence of MAP DNA in samples of artisanal coalho cheese purchased in the State of Pernambuco. Forty samples of coalho cheese submitted to the Real Time Polymerase Chain Reaction (qPCR) technique were analyzed for the detection of the MAP region IS900. 11 (27.5%) were positive with a mean of 195.9 MAP colony forming unit (CFU) per gram of each sample, with a minimum of 30.3... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine; IS900; QPCR; Paratuberculosis. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000601917 |
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| Santos,A.S.; Lima,D.C.V.; Fernandes,E.F.T.S.; Albuquerque,P.P.F.; Gouveia,G.V.; Sá,M.C.A.; Costa,M.M.; Pinheiro Júnior,J.W.; Mota,R.A.. |
| ABSTRACT Enteric diseases of bacterial origin are frequent in the pig industry, of particular notoriety are the colibacillosis that mainly affect piglets and cause great damage to the swine industry worldwide. The aim of the study was to analyze phylogenetics, to detect biofilm production, and to determine antimicrobial resistance profile in 126 strains of Escherichia coli isolated from swabs obtained from fragments of the small intestines of 235 healthy pigs killed in slaughterhouses in Pernambuco (Brazil) using polymerase chain reaction (PCR), adherence to microplates test and disc diffusion technique. Of the analyzed samples, 88.10% (111/126) were classified in phylogenetic group B1; 4.76% (6/126) in group D; 3.97% (5/126) in group B2 and, 3.17% (4/126)... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pig industry; Colibacillosis; Phylogeny; Antibiogram; Multi-resistance. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000401309 |
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